Research > Facilities > Orthopedic Molecular Medicine Suite and Applied Research Laboratory
This research suite is ~1000 ft2 of laboratory space with benchtop Internet-connected workstations. Room 325B is ~500 ft2 dedicated to DNA processing and gene expression analysis and contains two laminar flow hoods, a 80C freezer, an Iso Temp 20C freezer, refrigerator, Applied BioSystems Taqman 7000 Sequence Detection System (RT-PCR analyzer) and dedicated PC computer with Primer Express software, refrigerated Micro centrifuge, (2) MJ minicycler PCR Centrifuge engines, a Sorvall RC-5C Microcomputer Controlled Super speed Refrigerated Centrifuge with changeable rotors, New Brunswick Incubator/Shaker, Eppemdorf Electroporator 2510, electrophoresis gel-casting units, pH meter, analytical balance, water and dry baths, pipetters, supplies, glassware and other accessories necessary for molecular biology applications. Also in 325B, a central area houses a BioMate3 Spectrophotometer, Zylux Tube Luminometer, Kodak GelDoc Photography digital imaging system, and ultra Microplate reader served by a Dell 1.7 GHz processor. An iMac (1 Gb) computer with accessories including a digital still/video cam recorder, 6-card reader, and CD burning capabilities serves this section. This card reader serves as the computer interface for the digital image capture systems on the GelDoc, Olympus CK40 Inverted and CX41 Compound Microscope and Canon Elura 20 MC Video chip/digital camera. Room 325C is ~400 ft2 dedicated to cell/tissue culture and vector propagation and contains two laminar flow hoods, one that operates at BSL2-level containment. The module contains (2) ThermoForma water jacketed forced air, reach-in CO2 incubators, water bath, pipetters, refrigerator, Maytag dishwasher, Barnstead thermolyne autoclave, tissue homogenizer, liquid nitrogen storage tank, Olympus CK40 inverted florescent microscope with viewing screen, Olympus DP12 digital camera and Olympus P330N color printer. A power G3 computer serves this section. Room 325D is ~100 ft2 dedicated to imaging and processing and houses an Olympus CX41 phase-compound microscope with a camera and monitor, and a Zeiss stereomicroscope. This area is the processing center for the other modules and houses the common color printer, laser printer, and scanner. An iMac computer serves this module. Additionally the PI share an ~250 ft2 radioisotope laboratory equipped with tow laminar flow hoods, CO2 jacket incubator, and scintillation counter for cell/tissue culture metabolic studies.
Contact Information
Alicia L Bertone, DVM, PhD, Diplomate ACVS
Director of Research, Sports Medicine Center
Endowed Chair of Equine Clinical Medicine and Surgery, Department of Veterinary Clinical Sciences
380 VMAB
1900 Coffey Rd
Columbus, OH 43210
Phone: 614-292-6661
FAX: 614-688-5642
